PROTEIN CALCULATOR v3.4




TITLE:
SEQUENCE:

Note that the nonstandard "U" is selenocysteine.




OPTIONS:

Molecular Weight


Molecular Weight
Scan Mass Spectrometry Result:
      Target Mass

      All
      Trypsin
      Chymotrypsin
      V8 Protease

      Percent Difference
      Maximum Difference (Daltons)
      Min   Max : Mass Range (Daltons)

      Calculate masses of b+ and y+ daughter ions
Use Monoisotopic Masses (Not Isotopic Averages)
Methionine to Selenomethionine
Cysteine to Selenocysteine
Use 13C (monoisotopic and averaged isotope calculations)
Use 15N (monoisotopic and averaged isotope calculations)
Charge


Isoelectric Point (pI)
Charge at pH
List charges over pH range
  • Start pH=
  • End pH=
  • Step in pH=
Ultraviolet Absorbtion


Molar Absorbance
Absorbance in mg/mL
Atom and Residue Counting


Count Residues
Count Atoms
Solvent Content


Cell: a = b = c = alpha = beta = gamma =
Symmetry: number of symmetry operators (Z) =

Calculate for:

      Number in asymmetric unit (N) =
      Matthews Coeffient Range: start = end =
      Ignore Sequence and Use This Molecular Weight =




Last Updated: May 13, 2013